Sousa was born in Washington, D.C., to John António de Sousa and Maria Elisabeth Trinkhaus. His parents were of Portuguese, Spanish and Bavarian (German) descent; his grandparents were Portuguese refugees.
Sousa started his music education, playing the violin, as a pupil of John Esputa and G. F. Benkert for harmony and musical composition at the age of six. He was found to have absolute pitch. When Sousa reached the age of 13, his father, a trombonist in the Marine Band, enlisted his son in the United States Marine Corps as an apprentice. Sousa served his apprenticeship for seven years, until 1875, and apparently learned to play all the wind instruments while honing his mettle with the violin.
On December 30, 1879, he married Jane van Middlesworth Bellis. They had three children: John Philip Sousa, Jr (1 April 1881 - 18 May 1937), Jane Priscilla (7 Aug 1882 - 28 Oct 1958), and Helen (21 Jan 1887 - 14 Oct 1975). All three are buried in the John Philip Sousa plot in the Congressional cemetery. Jane joined the Daughters of the American Revolution in 1907.
Several years later, Sousa left his apprenticeship to join a theatrical (pit) orchestra where he learned to conduct. He returned to the U.S. Marine Band as its head in 1880, and remained as its conductor until 1892.
Sousa organized his own band the year he left the Marine Band. The Sousa Band toured 1892-1931, performing 15,623 concerts.
In 1900, his band represented the United States at the Paris Exposition before touring Europe. In Paris, the Sousa Band marched through the streets including the Champs-Élysées to the Arc de Triomphe – one of only eight parades the band marched in over its forty years.
Sousa repeatedly refused to conduct on the radio, fearing a lack of personal contact with the audience. He was finally persuaded to do so in 1929 and became a smash hit.
Sousa lived in Sands Point, New York. There is a school (John Philip Sousa Elementary), a band shell and a memorial tree planted in nearby Port Washington.
Sousa died on March 6, 1932, in his room at the Abraham Lincoln Hotel in Reading, Pennsylvania.
